Effect
Trainer treated Spinach, cv. Platypus RZ F1, showed significantly increased leaf number (no. plant−1) (+10.1%), increased leaf area (cm2 plant−1) (+31.8%), no difference in leaf dry matter (%) (-7.7%), increased yield (g m-2) (only significant at 0 and 15 kg N/ha), no difference in leaf dry weight (g m-2)(significant at 15 kgN/ha), no difference in SPAD index (+4.9%) or CIELAB colour space parameters, increased protein (+15.3%), no difference in nitrate (+22.8%), increased phosphorous (P) (+12.9%), no difference in potassium (K) (+5.4%), increased calcium (Ca) (+12.8%), increased magnesium (Mg) (+10.3%), no difference in sulphur (S) (-11.1%), no difference in sodium (Na) (+16.4%) content, no difference in starch content (-18.9%), decreased glucose content (-18.1%), no difference in fructose content (-11.4%), no differnce in carotenoids (-2.3%), decreased polyphenolcs content (-9.7%) in leaf tissue compared to untreated control plants, averaged across the different nitrogen (N) application rates of 0, 15, 30 or 45 kg ha-1.